ABSTRACT
Objective To determine the levels of plasma M-CSF in cervical cancer patients and cervical intraepitheliai neoplasia(CIN)patients,and to discuss the diagnostic utility of M-CSF in the development of cervi-cal cancer. Methods Forty-nine cases of cervical cancer,60 cases of CIN and 40 healthy controls were selected from June 2014 to June 2016. Plasma levels of M-CSF and SCC-Ag were determined by using ELISA and MEIA, respectively. Results Significantly different concentrations of M-CSF and SCC-Ag were observed in patients with cervical cancer compared to the healthy controls and CIN patients(P<0.01,respectively). Significant difference in plasma M-CSF level between the healthy controls and benign lesion patients were also found(P<0.05).However, no significant difference in SCC-Ag was found between the healthy controls and benign lesion patients. SCC-Ag combined M-CSF could obviously increase the detection rate of cervical cancer,without increasing the false posi-tive rate. The level of M-CSF in patients with cervical cancer and high grade of CIN reduced to the normal level, with significant difference before and after surgery(P<0.05).Meanwhile,the level of SCCAg in CIN patients was significantly reduced before and after surgery(P<0.05).Conclusions This study suggests the potential utility of M-CSF as a good candidate for a serum marker of cervical cancer,as well as benign lesions of this organ(CIN), and M-CSF may also be use to predict the outcome of CIN.
ABSTRACT
Further study on the chemical constituents of Artemisia sphaerocephala led to the isolation of fifteen compounds. These compounds were isolated and purified by repeated column chromatography on silica gel and Sephadex LH-20. Their structures were determined by physicochemical properties and spectral data analysis. These compounds were identified as 5-hydroxy-7,4'-dimethoxyflavanone (1), 5-hydroxy-7, 4'-dimethoxyflavone (2), 5-hydroxy-7, 4'-dimethoxyflavanonol (3), 5,3'-dihydroxy-7, 4'-dimethoxyflavanone (4), 5,7-dihydroxy-6,4'-dimethoxyflavone (5), isosakuranetin (6), hesperetin (7), navingenin (8), acacetin (9), chrysoeroil (10), 5,7-dihydroxy-4'-methoxyflavone-6,8-di-C- -glucopyranoside (11), didymin (12), acacetin-7-O-rutinoside (13), piceine (14), and capillarin (15). Compounds 1-3, 5, 7, 8, 10-15 were isolated from this plant for the first time.